Microscopy: Quantitative Analysis of Speckle Microscopy (Clare Waterman)

Fluorescent speckle microscopy is a technique that allows monitoring of dynamics in polymeric structures by doping in a very low level of fluorescently labeled monomer into the polymer. The small number of fluorescent molecules make fluorescent speckles that show up as diffraction-limited bright spots in the image. Here, Clare Waterman, developer of this technique, describes computational tools (developed by Gaudenz Danuser) for automatic quantitative analysis of speckle microscopy data.