Bright and faint bands - PCR or Gel electrophoresis fault?
Gel electrophoresis is a laboratory method used to separate mixtures of DNA, RNA, or proteins according to molecular size. In gel electrophoresis, the molecules to be separated are pushed by an electrical field through a gel that contains small pores.
Polymerase chain reaction (abbreviated PCR) is a laboratory technique for rapidly producing (amplifying) millions to billions of copies of a specific segment of DNA, which can then be studied in greater detail.
The following programs are good for spotting hair pin loops and primer dimers:
Create an IDT account and use the Oligo analyzer tool
Oligo calculator is really good at spotting self (Homo primer) annealing, e.g. hair pin loops
#gelElectrophoresis #Genetics #DNA #RNA #Proteins #rnase #protenase #useOfPCR #PCRTest #PCRExplained #PCRAnimation #PCRProcedure #PCRCovidTest #howPcrWorks #reverseTranscriptase #cDNA #stepsOfPcr #annealing #denaturing #DNASynthesis #nucleotides #pcrIngredients #purposeOfPcr #PCRSequence #dnaPolymerase #TaqPolymerase #amoebaSisters #amebaSisters #ameebaSisters #ameobaSisters #TEKS #biology #science #overview #NikolaysGeneticsLessons
1 view
11
5
1 week ago 00:36:25 1
Decree x40 “Beloved Surya“ by Elizabeth Clare Prophet
2 weeks ago 00:02:36 5
Jingle Bells | Christmas Song | Super Simple Songs