Dilution means to reduce the concentration of a solution.
A solution is produced when a solute dissolves in a solvent.
Concentration refers to the amount of solute dissolved in a volume of solution.
A solution can be diluted by adding more solvent to the solution, then
(i) volume of solution increases from V initial to V final
(ii) concentration of solution decreases from c initial to c final
Dilution factor refers to the ratio of the volume of the initial (concentrated) solution to the volume of the final (dilute) solution1,
that is, the ratio of V1 to V2
or
V1 : V2
A dilution factor, DF, can be calculated:
DF = V2 ÷ V1
Problem:
Your supervisor provides you with a 1.0 mg/mL solution of Bovine Serum Albumin (BSA), and asks you to make a 5-step 2-fold serial dilution. What would be the dilution factor at step 3?
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