In vivo imaging with multiphoton microscopy in developmental biology

Embryonic development of a zebrafish embryo. 3-channel overlay. Blue: RNA injection H2B; Red: mCherry; Green: EGFPRas. Conventional scanner: 200 Hz; Format: 512x512; 4D MP timelapse > 21h, 420 Stacks, Zebrafish embryo: Courtesy of Dr Nadine Peyrieras, CNRS, Gif sur Yvettes, France