How I Defeated My Arch Nemesis

For NordVPN’s 9th birthday, every purchase of a 2-year plan will get you 1 additional month free AND a surprise gift. Go to and use code thoughtemporium! ________________________________________________________________________ BL21 E. coli are by far the most obnoxious bacteria genetic engineers usually work with for 1 simple reason. They are maddeningly difficult to modify and load with new DNA. This wouldn’t be an issue if they weren’t also one of the best types for producing proteins en mass, and as such have application in everything from pharmaceutical production, to producing ingredients for foods. But after a few years of tinkering with it I finally found a protocol that reliably fixes this irritating issue, and makes it easy to transform them reliably. Written protocol - Sebastian’s spinner -