Mapping of Restriction Sites on Plasmid DNA

The Human Genome and other genome projects are extremely significant accomplish- ments with important applications to biology and medicine. The explosion of this new information is leading to dramatic changes in the way we are able to improve life. Part of the challenge in dealing with the enormous amounts of data is to determine what genes are responsible for different functions. Scientists must determine the location of genes through DNA mapping, and then begin the arduous task of determining what the individual genes do. Mapping the positions of restriction enzyme cleavage sites on a DNA molecule is an important prerequisite to DNA sequencing, which provides the primary nucleotide sequence information in DNA. Mapping involves the de- termination of the relative distances between restriction enzyme cleavage sites. An illustrative analogy would be somewhat similar to the following: If DNA mapping were compared to identifying the streets on a city map, then DNA sequencing would be analogous to identifying the specific houses on the streets. DNA mapping is performed by determining the size of the DNA fragments generated by single or combinations of restriction enzyme digestions, and subsequent construction of a DNA map. #NikolaysGeneticsLessons #restrictionSites #mappingRestrictionSites #plasmid #plasmidDNA #restrictionEnzymes #RestrictionMappingOfCircularDNA #restrictionMapping #howToMapRestrictionSites #RestictionEnzymeDigests #plasmids #apBiology #restrictionEnzyme #RestrictionEndonuclease #RestrictionMap #restrictionSite #palindromicSequence #RecombinationTechniques #PlasmidDigestion #gelElectrophoresis #RestrictionEnzymeDigest