Somatic cell hybridization

Human-Rodent Somatic Cell Hybrids - Mapping Human Chromosomes Somatic cell hybrids are culture lines that contain the entire complement of the mouse genome and a few human chromosomes. These culture lines are developed by mixing human and mouse cells in the presence of the Sendai virus. The virus facilitates the fusing of the two cell types to form a hybrid cell. For a reason that is not entirely known, most, but not all, human chromosomes are lost from the hybrid cell lines. Usually a few human chromosomes are retained. Because the human and mouse chromosomes can be distinguished by chromosome staining techniques, it can be determined which human cells are retained with a specific cell line. Typically, the mouse cell line is mutant for a specific function. Because the hybrids are selected on cells in which the mutant cells could not grow, any surviving hybrid lines would contain the human chromosome that complements (or rescues) the mouse mutation and several other human chromosomes. #SomaticCell #HybridOrganismClassificationRank #SomaticCellHybrids #cultureLines #hybridCell #chromosomes #CellAnatomicalStructure #NikolaysGeneticsLessons #SomaticCellHybridization #MarkerAssistedSelection #molecularBiology #plantBreeding #lifeSciences #LinkageMapping #GeneMapping #QuantitativeTraitLoci #BiochemicalMarker #RadiationHybridMapping #PhysicalMapping #recombinationFrequency #biologyEducation #GenotypicMarkers